Transient gene expression allows the rapid production of recombinant proteins.  Traditionally industry has utilised HEK cells for transient expression because of the lack of CHO cell transient systems which give high productivity. However CHO cells remain the host cell of choice for later stage commercial bioproduction using stable cell lines, and for this reason a high yielding CHO transient system would be desirable. 

At ACYTE Biotech we have developed a high-yielding CHO cell based transient gene expression system, avoiding any complications arising from changing host cells from early to late stage development.  This system, known as Epi-CHO is able to routinely produce titres of recombinant monoclonal antibodies exceedingly 100mg/L. 

Epi-CHO is able to achieve these high-yields through the use of proprietary genetic elements which allow transfected plasmid DNA to be replicated and retained during cell division. 

Epi-CHO consists of three main components:

• CHO-T: the production host cell line
• pEpiCHO: The expression vector
• Detailed transfection protocols for scalable production

For more information on Epi-CHO please see our recent publications.

Epi-CHO based transient protein production is available as a fee for service or the  Epi-CHO  system and protocols can be provided for use under license.  Please contact us for details.